Coding

Part:BBa_K5063016:Design

Designed by: Wong Chung Kiu   Group: iGEM24_HK-United   (2024-09-30)


miRFP670nano_TP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 865


Design Notes

His-tag is chosen as the affinity tag for our fusion protein due to its small size. This prevents the function or structure of our protein from being interfered. His-tags also provide inexpensive methods to perform purification.miRFP670nano has an excitation at 645 nm and emission at 670 nm, allowing to be detected by infrared. And for its advantages, it is highly stable to denaturation and degradation.



Source

[1] Leong, D. P., Joseph, P. G., McKee, M., Anand, S. S., Teo, K. K., Schwalm, J. D., & Yusuf, S. (2017). Reducing the global burden of cardiovascular disease, part 2: prevention and treatment of cardiovascular disease. Circulation research, 121(6), 695-710.https://www.ahajournals.org/doi/epub/10.1161/CIRCRESAHA.117.311849 [2] Loo, S., Kam, A., Dutta, B., Zhang, X., Feng, N., Sze, S. K., ... & Tam, J. P. (2024). Broad-spectrum ginsentides are principal bioactives in unraveling the cure-all effects of ginseng. Acta Pharmaceutica Sinica B, 14(2), 653-666.https://www.sciencedirect.com/science/article/pii/S2211383523004227 [3] Dutta B, Loo S, Kam A, Sze SK, Tam JP. Ginsentide TP1 Protects Hypoxia-Induced Dysfunction and ER Stress-Linked Apoptosis. Cells. 2023 May 16;12(10):1401. doi: 10.3390/cells12101401. PMID: 37408235; PMCID: PMC10216702.

References